H2N2V1, France, Analysis, bibRecord, 000196

Genetic reassortment of influenza viruses with different origins or subtypes

Identifieur interne : 000196 ( France/Analysis ); précédent : 000195; suivant : 000197

Genetic reassortment of influenza viruses with different origins or subtypes

Auteurs : Maude Bouscambert-Duchamp [France]

Source :

RBID : Hal:tel-00733681

Descripteurs français

mix :
- Génétique inverse, RT-PCR quantitative, Réassortiment génétique, Virus influenza.

English descriptors

mix :
- A(H1N1)2009, A(H5N1), Genetic reassortment, Influenza virus, Quantitative RT-PCR, Reverse genetic.

Abstract

In the context of A(H5N1) pandemics threat, an « avian flu and flu pandemics » project was proposed by LyonBioPole to develop influenza viruses characterization tools for vaccine production. To study genetic reassortment between influenza viruses, 3 reverse genetic systems of A(H3N2) human virus and A(H5N2) and A(H5N1) avian viruses were developed and reassortant viruses were produced. Their replicative capacities were evaluated using growth kinetics on MDCK cells with viral production quantification by real-time qRT-PCR. The A(H1N1)2009 emergence raises two questions about the acquisition by genetic reassortment of oseltamivir resistance and/or pathogenicity determinants. A co-infection protocol on MDCK cells was developed to study gene constellations of reassortant viruses like A(H1N1)2009-A(H1N1) H275Y and A(H1N1)2009-A(H5N1). We report here that genetic reassortment is possible between avian, human and swine strains using reverse genetic and viral co-infection and that some specific constellations emerged. We also report, that pandemic A(H1N1)2009 can acquire the H275Y mutated NA from seasonal oseltamivir resistant A(H1N1) viruses without any modifications on replicative capacities. This genetic reassortment is also possible with A(H5N1) viruses. These observations strenght the importance of vaccination against all these influenza strains to reduce the risk of one-individual viral co-infection.

Url:

https://tel.archives-ouvertes.fr/tel-00733681

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000118
to stream Hal, to step Curation: 000118
to stream Hal, to step Checkpoint: 000259
to stream Main, to step Merge: 000D82
to stream Main, to step Curation: 000D76
to stream Main, to step Exploration: 000D76
to stream France, to step Extraction: 000196

Links to Exploration step

Hal:tel-00733681

Le document en format XML

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<front><div type="abstract" xml:lang="en">              <p>In the context of A(H5N1) pandemics threat, an « avian flu and flu pandemics » project was proposed by LyonBioPole to develop influenza viruses characterization tools for vaccine production. To study genetic reassortment between influenza viruses, 3 reverse genetic systems of A(H3N2) human virus and A(H5N2) and A(H5N1) avian viruses were developed and reassortant viruses were produced. Their replicative capacities were evaluated using growth kinetics on MDCK cells with viral production quantification by real-time qRT-PCR. The A(H1N1)2009 emergence raises two questions about the acquisition by genetic reassortment of oseltamivir resistance and/or pathogenicity determinants. A co-infection protocol on MDCK cells was developed to study gene constellations of reassortant viruses like A(H1N1)2009-A(H1N1) H275Y and A(H1N1)2009-A(H5N1). We report here that genetic reassortment is possible between avian, human and swine strains using reverse genetic and viral co-infection and that some specific constellations emerged. We also report, that pandemic A(H1N1)2009 can acquire the H275Y mutated NA from seasonal oseltamivir resistant A(H1N1) viruses without any modifications on replicative capacities. This genetic reassortment is also possible with A(H5N1) viruses. These observations strenght the importance of vaccination against all these influenza strains to reduce the risk of one-individual viral co-infection.</p>
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   |wiki=    Sante
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   |clé=     Hal:tel-00733681
   |texte=   Genetic reassortment of influenza viruses with different origins or subtypes
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Genetic reassortment of influenza viruses with different origins or subtypes

Genetic reassortment of influenza viruses with different origins or subtypes

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri